Comparation of two anti-EphA2 CAR-T constructs. Comparation of two anti-EphA2 CAR-T constructs

We constructed two different third generation anti-EphA2 chimeric antigen receptors(CARs) for glioblastoma treatment. They have the same transmembrane and intracellular domains. The only different is the extracellular domain. T cells transduced with these two different CARs exhibited different anti-tumor capacities and cytokine secretion. So RNA-seq was used to anaylze the different gene expression archetecture of these two CAR-T cells. Overall design: Compare two CAR-T cells gene expression profile

本研究构建了两种不同的第三代抗EphA2嵌合抗原受体（chimeric antigen receptor, CAR），用于胶质母细胞瘤的治疗。二者具备完全一致的跨膜结构域与胞内结构域，唯一的差异仅存在于胞外结构域。转导了这两种CAR的T细胞展现出不同的抗肿瘤能力与细胞因子分泌特性，因此本研究采用RNA测序（RNA-seq）技术，分析这两种CAR-T细胞的基因表达架构差异。实验整体设计：对比两种CAR-T细胞的基因表达谱。