Human dendritic cells treated with Echinacea purpurea phytocompounds. Homo sapiens

This study investigated the specific and differential gene expression in human immature DCs (iDCs) in response to treatment with a butanol fraction containing defined bioactive phytocompounds extracted from stems and leaves of Echinacea purpurea Overall design: Human immature DCs (iDCs) differentiated from primary monocytes in vitro were incubated with [BF/S+L/Ep] or cichoric acid for 4 or 24 h to characterize early- or late-responsive genes. A total of nine Affymetrix HU-133A chips were hybridized to determine the transcriptome profiles in human iDCs.本研究探究了人类未成熟树突状细胞（immature DCs, iDCs）经含从紫锥菊（Echinacea purpurea）茎叶中提取的明确生物活性植物成分的正丁醇萃取组分（butanol fraction）处理后的特异性与差异基因表达特征。总体设计：将体外由原代单核细胞（primary monocytes）分化获得的人类未成熟树突状细胞，分别以[BF/S+L/Ep]或菊苣酸（cichoric acid）孵育4小时或24小时，以此鉴定早期响应基因与晚期响应基因。本研究共使用9张Affymetrix HU-133A基因芯片开展杂交实验，以解析人类iDCs的转录组谱（transcriptome profiles）。