HOT-288 SKQ201615S Fluorometric Chlorophyll

These Simons Collaboration on Ocean Processes and Ecology (SCOPE) data products were produced by the University of Hawaiʻi at Mānoa with support from the Simons Foundation. The HOT-288 cruise on the R/V Sikuliaq was performed from November 25th to 29th, 2016 at Station ALOHA (22.75, -158) north of Oahu, Hawaii. Fluorometric chlorophyll samples were collected from rosette niskin bottles at specific depths and filtered at low vacuum through a glass fiber filter. Samples were then flash frozen in liquid nitrogen and stored in a histoprep capsule at -80°C until analysis. Post-cruise, the filters were extracted using 90% acetone for a total of 48 hours in a -20°C freezer, covered to prevent the photodegradation of pigments. Upon extraction, samples were centrifuged to settle filter particulates and gently poured into a glass cuvette. Samples were then run on a Turner 10-AU Fluorometer. Data was corrected for average glass fiber filter blank values and is reported as the average of triplicate samples. Sample with no cast/niskin was collected from underway flowthrough system. The timestamp is in UTC.本数据集属于西蒙斯海洋过程与生态合作研究（Simons Collaboration on Ocean Processes and Ecology, SCOPE）的数据产品，由夏威夷大学马诺阿分校（University of Hawaiʻi at Mānoa）制作完成，研究工作得到西蒙斯基金会（Simons Foundation）的资助。 2016年11月25日至29日，“西库利亚克号”（R/V Sikuliaq）科考船执行了HOT-288航次，作业站位位于夏威夷瓦胡岛以北的ALOHA站（22.75, -158）。研究人员从特定深度的罗塞特式尼辛采水瓶（rosette niskin bottles）中采集荧光法叶绿素样品，经低真空过滤至玻璃纤维滤膜（glass fiber filter）上。随后将滤膜置于液氮中快速冷冻，装入组织包埋胶囊（histoprep capsule），并于-80℃环境下保存直至开展分析。航次结束后，研究人员将滤膜置于-20℃避光冰箱中，用90%丙酮萃取48小时，全程避光以避免色素发生光降解。萃取完成后，对样品进行离心以沉降滤膜颗粒物，随后小心将上清液转移至玻璃比色皿（glass cuvette）中。使用Turner 10-AU型荧光光度计（Turner 10-AU Fluorometer）对样品进行荧光检测。所有检测数据已扣除玻璃纤维滤膜的平均空白值，最终结果以三份平行样品的平均值进行报告。另有一份无对应站位/采水瓶的空白样品，由走航流通系统（underway flowthrough system）采集得到。所有时间戳均采用协调世界时（UTC）格式。