Expression profiling of Natural killer B cells

Investigation of global gene expression levels between B cells, Natural killer cells and Natural killer B cells Gene expression profiling using sorted B cells, Natural killer cells and Natural killer B cells from WT mouse spleen. Total RNA extracted from WT cells were quantified by the NanoDrop ND-1000 and RNA integrity was assessed by standard denaturing agarose gel electrophoresis. The sample preparation and microarray hybridization were performed based on the NimbleGen’s standard protocols.本研究针对B细胞、自然杀伤细胞（Natural Killer Cells）与自然杀伤B细胞（Natural Killer B Cells）的全基因表达水平展开分析：实验采用从野生型（Wild Type, WT）小鼠脾脏中分选得到的B细胞、自然杀伤细胞与自然杀伤B细胞进行基因表达谱检测；从上述野生型细胞中提取的总RNA通过NanoDrop ND-1000完成定量，并通过标准变性琼脂糖凝胶电泳评估其完整性；样本制备与微阵列杂交实验均依照NimbleGen的标准实验规程实施。