Transcriptome changes in a rat model of progressive heart failure. Transcriptome changes in a rat model of progressive heart failure

Heart failure is among the leading causes of death globally. Ventricular failure progresses through a hypertrophic compensatory phase followed by failure of the ventricle function through rapid decompensation. In order to unravel right heart specific mechanisms of disease, rat animal models were established that (i) reflect the slowly progressive mode of compensation / decompensation and (ii) allow comparative analyses of left versus right heart failure in the same experimental set up. Differential gene expression analysis was performed for all three treatment groups (sham, AOB, PAB), at both time points (compensation, decompensation) and for both ventricles as well as the septum Overall design: From both phases of AOB and PAB, as well as from matching sham-operated animals, whole heart tissues were isolated and total RNA was extracted from the left ventricle, from the right ventricle and from the septum area. Then, polyA mRNAseq was performed. A total of 33 animals were included in this study.

心力衰竭是全球范围内的主要致死病因之一。心室衰竭的进展通常先经历肥厚性代偿阶段，随后因快速失代偿而出现心室功能衰竭。为阐明右心特有的疾病发病机制，研究人员构建了大鼠动物模型，该模型可实现两大特点：（i）模拟缓慢进展的代偿/失代偿过程；（ii）在同一实验体系中开展左心与右心衰竭的对比分析。本研究针对三个处理组（假手术组（sham）、AOB组、PAB组）、两个时间节点（代偿期、失代偿期）的样本，以及左心室、右心室与室间隔组织开展了差异基因表达分析。实验设计：从AOB模型组、PAB模型组以及匹配的假手术动物体内分离全心脏组织，分别从左心室、右心室及室间隔区域提取总RNA，随后进行polyA信使RNA测序（polyA mRNAseq）。本研究共纳入33只实验动物。