Expression data from tomato root tissue inoculated or not with a plant growth promoting bacteria (PGPB) strain

Possitive effects of plant growth promoting bacteria (PGPB) inoculation on plant growth and development are dependent on interaction between bacterial strains and plant roots, which are usually the bacterial niche. Furthermore, phytohormones are key regulators of plant physiology. Ethylene is essential in plant growth and development and in response to drought. Plant sensibility to ethylene is involved in plant response to PGPB strain inoculation and plant growth promotion. We used microarrays to detail the global programme of gene expression underlying plant interaction with two different PGPB strains (isolated from arid soils in southern Spain) regarding to plant sentitivity to ethylene by tomato ethylene receptor 3 (SlETR3). Ethylene-insensitive never ripe and its isogenic wild-type cv. Pearson lines were inoculated with either Bacillus megaterium or Enterobacter sp. C7 and grown until mature stage (eight weeks post-inoculation) under well-watered and drought conditions. The present study pursued to assess the bacterial effects on gene expression in tomato (Solanum lycopersicum). Root tissue samples inoculated or not with a bacterial strain were selected for RNA extraction and hybridization on Affymetrix microarrays. To that end, we selected three samples per treatment according to plant sensitivity to ethylene, watering regime and inoculated bacteria: wild type cv Pearson (WT) and never ripe (NR) plants under well-watered and drought (D) conditions non-inoculated (NO, controls), or inoculated either with Bacillus megaterium (BM), or Enterobacter C7 (C7).

植物促生细菌（Plant Growth Promoting Bacteria，PGPB）接种对植物生长发育的正向调控效应，依赖于细菌菌株与植物根系（通常为细菌的定殖生态位）之间的互作。此外，植物激素是植物生理过程的核心调控因子。乙烯在植物生长发育以及干旱胁迫应答过程中均发挥不可或缺的作用，而植物对乙烯的敏感性参与了其响应PGPB菌株接种并获得生长促生效应的过程。本研究利用基因芯片技术，解析了番茄乙烯受体3（SlETR3）介导的乙烯敏感性背景下，植物与两株分离自西班牙南部干旱土壤的不同PGPB菌株互作时的全局基因表达调控网络。本实验选取乙烯不敏感突变体never ripe及其等基因野生型Pearson品种植株，分别接种巨大芽孢杆菌（Bacillus megaterium）或肠杆菌属菌株C7（Enterobacter sp. C7），随后在正常供水与干旱胁迫条件下培养至成熟阶段（接种后8周）。本研究旨在探究PGPB对番茄（Solanum lycopersicum）基因表达的调控效应。我们选取接种或未接种细菌菌株的根系组织样本，进行RNA提取并在Affymetrix基因芯片上完成杂交实验。为此，我们根据植物对乙烯的敏感性、供水处理以及接种的细菌菌株，为每个实验处理设置3个生物学重复样本，具体分组如下：正常供水与干旱胁迫下未接种的野生型Pearson（WT）和never ripe（NR）植株（作为对照组，标记为NO），以及分别接种巨大芽孢杆菌（BM）或肠杆菌C7（C7）的对应植株组。