Additional file 1 of A new strain of Neowestiellopsis (Hapalosiphonaceae): first observation of toxic soil cyanobacteria from agricultural fields in Iran

Additional file 1: Supplementary Fig. S1. Life cycle of the new Neowestiellopsis ca. persica isolated from Iran. a) Hormogonium, b) filament from hormogonium, c) Akinets, d) Akinets germination, e) multiseriate filament from akinets, f) Monocyte formation from the ends of filaments, g) Monocyte with heterocyte, h) little heteropolar filaments from Monocites, i) heteropolar filaments, j) typical Neowestiellopsis filament. Supplementary Fig. S2. Phylogenetic position of studied strain (Highlighted in red) and related cyanobacteria based on mcy G gene with Microcystis aeruginosa (AB110133) as out-group. Numbers near nodes indicate standard bootstrap support (%)/ultrafast bootstrap support (%) for ML analyses. Supplementary Fig. S3. Phylogenetic position of studied strain (Highlighted in red) and related cyanobacteria based on mcy D gene with PCC_7806SL (CP020771) as out-group. Numbers near nodes indicate standard bootstrap support (%)/ultrafast bootstrap support (%) for ML analyses. Supplementary Fig. S4. Phylogenetic position of studied strain (Highlighted in red) and related cyanobacteria based on nos gene. Numbers near nodes indicate standard bootstrap support (%)/ultrafast bootstrap support (%) for ML analyses. Table S1. Target genes and oligonucleotide primers used in this study. Table S2. Accession numbers of Sequence data deposited in the DNA Data Bank of Japan. Table S3. Comparison of the nucleotides length of the ITS regions of Neowestiellopsis persica with reference strains. Table S4. Comparison of secondary structure of 16S-23S rRNA (D1-D1, helix and Box-B helix) between the Neowestiellopsis persica and related taxa. Table S5. Comparison of secondary structure of 16S-23S rRNA (D2, helix and V3) between the Neowestiellopsis persica and related taxa.

附加文件1：补充图S1。从伊朗分离的新型近似波斯新威斯特藻（Neowestiellopsis ca. persica）的生活史。a）藻殖段（Hormogonium），b）藻殖段衍生的丝状体，c）厚壁休眠孢子（Akinetes），d）厚壁休眠孢子萌发，e）由厚壁休眠孢子萌发形成的多列丝状体，f）丝状体末端形成单孢子（Monocyte），g）带有异形胞的单孢子，h）由单孢子产生的微弱异极性丝状体，i）异极性丝状体，j）典型的新威斯特藻丝状体。补充图S2。基于mcy G基因的供试菌株（以红色高亮标注）与相关蓝细菌的系统发育位置，以铜绿微囊藻（Microcystis aeruginosa，AB110133）作为外类群。节点旁的数值表示最大似然（ML）分析的标准自展支持率（%）/超快速自展支持率（%）。补充图S3。基于mcy D基因的供试菌株（以红色高亮标注）与相关蓝细菌的系统发育位置，以PCC_7806SL（CP020771）作为外类群。节点旁的数值表示最大似然（ML）分析的标准自展支持率（%）/超快速自展支持率（%）。补充图S4。基于nos基因的供试菌株（以红色高亮标注）与相关蓝细菌的系统发育位置。节点旁的数值表示最大似然（ML）分析的标准自展支持率（%）/超快速自展支持率（%）。表S1。本研究使用的靶基因与寡核苷酸引物。表S2。提交至日本DNA数据银行（DNA Data Bank of Japan）的序列数据登录号。表S3。波斯新威斯特藻（Neowestiellopsis persica）ITS区域核苷酸长度与参考菌株的比较。表S4。波斯新威斯特藻与相关类群的16S-23S rRNA（D1-D1结构域、螺旋区与Box-B螺旋）二级结构比较。表S5。波斯新威斯特藻与相关类群的16S-23S rRNA（D2结构域、螺旋区与V3环）二级结构比较。