MND_BACs. MND_BACs

BACs spanning FP contigs harbouring a single gene fragment(HvMND_F/R4) and the closely related flanking markers were identified by screening a BAC library representing all clones of the minimum-tiling path of the genome-wide physical map of barley (IBSC, 2012) and were sequenced subsequently using NGS technology.通过筛选涵盖大麦全基因组物理图谱（国际大麦测序联盟，International Barley Genome Sequencing Consortium，IBSC，2012）最小叠瓦路径全部克隆的细菌人工染色体（Bacterial Artificial Chromosome，BAC）文库，成功鉴定得到一类细菌人工染色体克隆，此类克隆跨越携带有单个基因片段（HvMND_F/R4）以及与其紧密相关侧翼标记的FP重叠群（FP contigs）；后续采用下一代测序（Next-Generation Sequencing，NGS）技术对上述BAC克隆进行了测序。