Sections of eyes with clinically diagnosed AMD immunostained for ELAVL1/HuR.

The immunopositivity of the nuclei of RPE cells (shown by arrows) and the extent of immunopositivity in the drusen (asterisks) was evaluated microscopically (no staining or positive staining) by selecting 5 mm long areas of foveomacular (A), perimacular (B) and peripheral (C) regions. The foveomacular nuclei showed less extensive immunopositivity for ELAVL1/HuR than the perimacular and peripheral regions. However this difference was not statistically significant in any of the groups studied (p>0.1). The cytoplasms of RPE cells were ELAVL1/HuR negative. Most of the drusen were ELAVL1/HuR-negative. (Original magnifications of x 200 and in insets x 400; Bruch's membrane shown by arrow heads).

本研究选取黄斑中心凹区（A组）、黄斑周区（B组）及视网膜周边部（C组）5mm长的视野区域，以无染色或阳性染色为评判标准，在显微镜下评估了视网膜色素上皮（Retinal Pigment Epithelium, RPE）细胞核（箭头标示）的免疫阳性状态，以及玻璃膜疣（drusen，星号标示）的免疫阳性范围。结果显示，黄斑中心凹区的细胞核对ELAVL1/HuR的免疫阳性程度弱于黄斑周区与视网膜周边部，但该差异在所有研究组中均无统计学意义（p>0.1）。视网膜色素上皮细胞的胞浆呈ELAVL1/HuR免疫阴性，绝大多数玻璃膜疣亦为ELAVL1/HuR免疫阴性。（原始放大倍数：×200，插图放大倍数：×400；布鲁赫膜（Bruch's membrane）由箭头头标示）