USE OF ALTERNATIVE EXTENDERS AND TEMPERATURES IN LONG TERM STORAGE OF BOAR SEMEN

Abstract The use of appropriate extenders is important for the success of an artificial insemination program. The objective of this study was to evaluate the efficiency of alternative extenders for swine semen at different temperatures (17 to 10 °C). The following extenders were used: Beltsville Thawing Solution (BTS), powdered coconut water (ACP-103®), and skimmed milk powder (LPD). The 50 ejaculates were analyzed daily, in natura and after dilution, during the 5-day period of semen preservation (D0 to D4), regarding spermatic vigor and motility. Acrosome integrity and sperm viability were evaluated on D0 and D4. Data were analyzed using Mann-Whitney, Students, Tukey and chi-square tests (p<0.05). The LPD extender at 10 °C presented higher motility and sperm vigor compared to BTS and ACP until D2, and to treatments stored at 17 °C. Acrosome vitality and integrity remained higher (p<0.001) with LPD at 10 °C on D0 and D4. LPD showed to be a good extender for the swine semen at lower temperature (10 °C). Furthermore, it provided better protection to sperm cells, by allowing greater integrity and vitality of the acrosome.

摘要 选用适配的稀释液对人工授精项目的顺利开展至关重要。本研究旨在评估不同温度（17℃至10℃）条件下猪精液替代稀释液的应用效果。本次试验采用的稀释液包括：贝尔茨维尔解冻液（Beltsville Thawing Solution，BTS）、粉状椰子水（ACP-103®）以及脱脂奶粉（LPD）。在为期5天的精液保存周期（D0至D4）内，每日对50份鲜精样本及稀释后样本进行精子活力与运动性能检测；分别于D0与D4时间点检测精子顶体完整性与精子存活率。数据采用曼-惠特尼检验、学生t检验、图基检验以及卡方检验进行统计分析，检验水准设定为p<0.05。结果显示，在保存至D2时，10℃环境下的LPD稀释液组的精子运动性能与活力均优于BTS组、ACP-103®组，同时优于17℃保存的所有处理组。在D0与D4时间点，10℃环境下的LPD稀释液组的精子顶体存活率与完整性均维持在更高水平（p<0.001）。研究证实，LPD可作为低温（10℃）环境下猪精液保存的优质稀释液，其能为精子细胞提供更优保护，有效维持精子顶体的完整性与存活率。