Expression data from human monocyte derived macrophages infected with adenovirus expressing HIV-1 Vpr

HIV-1 Vpr protein is a multifunctional protein which perturbs human transcriptome and interacts with a number of cellular proteins. In this study, we have attempted to explore the efffects of Vpr on human transcriptome and have identified several genes which are involved in innate immune respone and cell signaling pathways. We used the microarray analysis to elucidate the differnetail expression pattern of differnet genes in human macrophages infected with HIV-1 Vpr. As result we found that HIV-1 Vpr protein leads to the induction of various interferon stimualted genes (ISGs) and chemokines in human macrophages. Human monocytes-derived macrophages (MDMs) were isolated from peripheral blood mononuclear cells (PBMCs) from two healthy donors and were infected with recombinant adenoviruses either expressing HIV-1 Vpr or ZsGreen1 as a control. At 48 hours post-infection, RNA was isolated and subjected to microarray analysis.

HIV-1 Vpr蛋白是一种多功能蛋白，可扰乱人类转录组并与多种细胞蛋白相互作用。本研究旨在探究Vpr对人类转录组的影响，已鉴定出多个参与先天免疫应答与细胞信号通路的基因。本研究采用基因微阵列分析（microarray analysis），解析了感染HIV-1 Vpr的人类巨噬细胞中各基因的差异表达谱。结果发现，HIV-1 Vpr蛋白可诱导人类巨噬细胞中多种干扰素刺激基因（interferon stimulated genes, ISGs）与趋化因子（chemokines）的表达。研究人员从两名健康供体的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）中分离出单核细胞来源的巨噬细胞（monocytes-derived macrophages, MDMs），分别用表达HIV-1 Vpr的重组腺病毒及作为对照的ZsGreen1重组腺病毒进行感染。感染后48小时，提取RNA并进行基因微阵列分析。