Gene expression analysis of mESCs following short-term inhibition with PD032, CHIRON, EPZ6438 and GSK-J4 both individually and in combination.. Mus musculus

This experiment was designed to determine the extent of gene misregulation in mouse ESCs cultured for 24hrs in different base medias and treated with the indicated inhibitors. Overall design: Polyadenylated mRNA from mESCs cultured in the indicated culture conditions was hybridised as three independed biological replicates to Agilent SurePrint G3 Mouse GE 8x60K Microarrays (GPL10787). Individual replicates for each of the specified conditions were performed in HV H2b-mCh, HV9.3 and HFHC12.3 cell lines.

本实验旨在探究在不同基础培养基中培养24小时并经指定抑制剂处理的小鼠胚胎干细胞（mouse ESCs）的基因失调程度。实验整体设计：将在指定培养条件下培养的小鼠胚胎干细胞的聚腺苷酸化mRNA，以3次独立生物学重复的方式与安捷伦SurePrint G3小鼠基因表达8x60K微阵列（GPL10787）进行杂交。各指定条件下的独立重复实验，均在HV H2b-mCh、HV9.3及HFHC12.3细胞系中完成。