Effects of drought stress on root space microbial diversity of spring wheat

The field experiment was initiated in 2022 at Inner Mongolia Autonomous Region Academy of Agriculture and Animal Husbandry Tenihe Soil Management and Ecological Restoration Scientific Observation and Experimental Station(Tenihe Farm), Inner Mongolia Autonomous Region, China (650 m above sea level).Endosphere, rhizosphere, and bulk soil bacteria and fungi were sequenced using 16S rRNA and ITS amplicon sequencing techniques. A total of 162 samples (18 treatments *3 sample types (root, rhizosphere, and bulk soil)*3 replicates =162) were collected

本田间试验于2022年在中国内蒙古自治区农牧科学院特尼河土壤管理与生态修复科学观测实验站（特尼河农场）启动，该观测站海拔650米。本研究采用16S rRNA和ITS扩增子测序技术，对内生圈（endosphere）、根际（rhizosphere）及非根际土壤（bulk soil）中的细菌与真菌进行测序分析。本次试验共收集162份样本，具体组成为18个处理组×3种样本类型（根样本、根际土壤、非根际土壤）×3次生物学重复，共计162份样本。