The miRNA-200 family and miRNA-9 exhibit differential expression in primary vs. corresponding metastatic tissue in breast cancer

Global miRNA expression profiling was performed on 47 tumor samples from 14 patients with paired samples from primary breast tumors and corresponding lymph node and distant metastases using LNA-enhanced miRNA microarrays. The identified miRNA expression alterations were validated by real-time PCR, and tissue distribution of the miRNAs was visualized by in situ hybridization Global miRNA expression profiling was performed on 47 tumor samples from 14 patients with paired samples from primary breast tumors and corresponding lymph node and distant metastases using LNA-enhanced miRNA microarrays. The identified miRNA expression alterations were validated by real-time PCR, and tissue distribution of the miRNAs was visualized by in situ hybridization

本研究采用锁核酸（LNA）增强的微小RNA（miRNA）微阵列，对14名患者的47份肿瘤样本开展全微小RNA（miRNA）表达谱分析，所涉样本均包含原发性乳腺肿瘤及其配对的淋巴结转移灶与远处转移灶。通过实时聚合酶链反应（real-time PCR）对鉴定得到的微小RNA（miRNA）表达差异进行验证，并借助原位杂交（in situ hybridization）实现微小RNA（miRNA）的组织分布可视化。本研究采用锁核酸（LNA）增强的微小RNA（miRNA）微阵列，对14名患者的47份肿瘤样本开展全微小RNA（miRNA）表达谱分析，所涉样本均包含原发性乳腺肿瘤及其配对的淋巴结转移灶与远处转移灶。通过实时聚合酶链反应（real-time PCR）对鉴定得到的微小RNA（miRNA）表达差异进行验证，并借助原位杂交（in situ hybridization）实现微小RNA（miRNA）的组织分布可视化。