Methionine biosynthesis enzyme MoMet2 is required for rice blast fungus pathogenicity by promoting virulence gene expression via reducing 5mC modification. Methionine biosynthesis enzyme MoMet2 is required for rice blast fungus pathogenicity by promoting virulence gene expression via reducing 5mC modification

To investigate the function of Momet2 in Magnaporthe oryzae, we generated Momet2 mutant. To understand the impact of MoMET2-depentent 5mC to gene expression at the transcriptional level, we performed comparative transcriptome analysis using the materials harvested from Guy11 and ΔMomet2 mycelium samples. Overall design: Comparative gene expression profiling analysis of RNA-seq data for Guy11 and Momet2 mutant mycelium samples

为探究稻瘟病菌（Magnaporthe oryzae）中Momet2的功能，我们构建了Momet2突变体。为解析MoMET2依赖型5-甲基胞嘧啶（5mC）在转录水平对基因表达的调控效应，我们以野生型菌株Guy11与ΔMomet2菌丝体样本为实验材料，开展了比较转录组分析。整体实验设计：针对野生型Guy11与Momet2突变体菌丝体的RNA测序（RNA-seq）数据开展基因表达谱比较分析。