rtWIZARD protocol trial runs.

Lateral flow rapid diagnostic tests (RDTs, RTs) are cost-effective with low infrastructure requirements for limited-resource settings, and in any setting can represent a bridge between early disease monitoring at outbreak onset and fully-scaled molecular testing for human or animal diseases. However, the potential of RTs to handle higher throughput testing is hampered by the need for manual processing. Here we review dengue virus and African swine fever virus rapid tests, and present a novel protocol that employs an open-source fluid handler to automate the execution of up to 42 RTs per run. A publicly accessible website, rtWIZARD.lji.org, provides printouts for correctly spacing cassettes, worksheets for sample organization, and test-specific fluid handler protocols to accurately deliver samples from a 48-tube rack to each cassette’s sample and running buffer wells. An optional QR-coded sheet allows for de-identified sample-to-result traceability by producing a unique printable label for each cassette, enabling results to be entered via a scanner. This work describes a highly cost-effective model for increasing outbreak diagnostic efficiency and of increasing RT throughput for other applications including workplace testing, food safety, environmental testing, and defense applications.侧流快速诊断检测（Lateral flow rapid diagnostic tests，简称RDTs、RTs）具备成本效益高、基础设施需求低的优势，适用于资源匮乏场景；在各类场景中，均可作为疫病暴发初期监测与针对人类或动物疾病的规模化分子检测之间的桥梁。然而，RTs的高通量检测潜力受限于其依赖人工操作的瓶颈。本研究综述了登革病毒与非洲猪瘟病毒快速检测试剂，并提出一种新型实验方案：采用开源流体处理装置，可实现单次运行最多自动化处理42份RTs检测。公开访问网站rtWIZARD.lji.org可提供检测卡正确间距的打印模板、样本整理用的记录表单，以及适配特定检测的流体处理操作方案，可精准将48管架中的样本添加至每块检测卡的样本孔与运行缓冲液孔中。可选配的二维码表单可通过为每块检测卡生成唯一可打印标签，实现无标识化的样本至结果全流程可追溯，同时支持通过扫描仪录入检测结果。本研究提出了一款高性价比方案，可提升疫病暴发诊断效率，同时可为职场检测、食品安全检测、环境检测及国防应用等其他场景提升RTs检测通量。