Pathways associated with ATP production in lymphoblasts from ALS patients with mutant SOD1 (mutSOD1) or unknown mutation (undSOD1) and respective controls.

Cells were plated at density 160,000 cells/well, as described in Materials and Methods. Oxygen consumption rate (OCR) and Extracellular Acidification Rate were monitored over time using the Seahorse XFe96 Extracellular Flux Analyzer and the ATP Real-Time rate assay. Data were plotted for all cohorts (pool), C1-Female 46 years old, C2- Male 46 years old and C3-Male 26/27 years old. Several bioenergetic parameters were evaluated.

按照《材料与方法》中记载的实验步骤，以每孔160,000个细胞的密度接种细胞。采用Seahorse XFe96细胞外流量分析仪与ATP实时速率检测实验，实时监测细胞的耗氧率（Oxygen Consumption Rate, OCR）与细胞外酸化率。针对全部队列（混合组）、C1组（46岁女性）、C2组（46岁男性）以及C3组（26/27岁男性）的数据进行可视化绘图。本研究共评估了多项细胞能量代谢相关参数。