RNA seq of tomato hairy root cultures induced with nuclear-lokalized effectors of R. irregularis

In this research we have made tomato hairy roots expressing R. irregularis nuclear-lokalized effector proteins, RiSP749, GLOIN707, GLOIN781, and GFP as a control, under the XVE promoter. Hairy roots were cultured for 3 weeks after which the expression of the effectors was induced with estradiol. 24 h after estradiol treatment, roots were harvested and RNA was used for RNA sequening

本研究构建了在XVE启动子（XVE promoter）调控下，分别表达根内球囊霉（R. irregularis）核定位效应蛋白RiSP749、GLOIN707、GLOIN781，以及作为阴性对照的绿色荧光蛋白（GFP，Green Fluorescent Protein）的番茄毛状根。将上述毛状根培养3周后，利用雌二醇（estradiol）诱导效应蛋白的表达。雌二醇处理24小时后，收取根系并提取RNA，用于RNA测序。