Study of the mycobiota of the canine interdigital fold with pododermatitis by next generation sequencing

The yeast Malassezia pachydermatis is a common inhabitant of the skin and mucosae of dogs. However, under certain circumstances the yeast can overgrow and act as a secondary pathogen causing otitis and dermatitis in dogs. Canine pododermatitis is a common disease in dogs in which M. pachydermatis acts as a secondary pathogen. In the present study, the presence of Malassezia was assessed and quantified by different methods like cytological examination, culture, qPCR, and next generation sequencing (NGS). Samples from dogs with pododermatitis, healthy dogs and dogs after treatment were included.

厚皮马拉色菌（Malassezia pachydermatis）是犬皮肤与黏膜的常见定植酵母菌。但在特定条件下，该菌可过度增殖，成为犬的继发条件致病菌，引发中耳炎与皮炎。犬足皮炎是犬类常见疾病，厚皮马拉色菌在此病中可作为继发致病菌。本研究通过细胞学检查、微生物培养法、实时荧光定量PCR（qPCR）以及高通量测序（next generation sequencing, NGS）等多种方法，对马拉色菌属（Malassezia）的存在情况进行检测与定量分析。本研究纳入的样本包括患足皮炎的犬、健康犬以及接受治疗后的犬。