B.thetaiotaomicron mono-association versus B. thetaiotaomicron co-colonization with B. longum, PR chow, B6

Genome wide transcriptional comparison of B. thetaiotaomicron mono-association vs. B. thetaiotaomicron bi-association with B. longum. Cecal populations from 10d colonizations with b6 gnotobiotic mice fed a standard-chow polysaccharide rich (PR) diet (n=5 samples/group) were profiled. Keywords: Mono-association vs. Bi-association, in vivo Total RNA was prepared from cecal contents of 10 day associated gnotobiotic mice and hybridized to B. thetaiotaomicron/B. longum GeneChips.

本数据集针对脆弱拟杆菌（Bacteroides thetaiotaomicron）单菌定植与脆弱拟杆菌联合长双歧杆菌（Bifidobacterium longum）双菌定植的样本开展全基因组转录组比较分析。实验选取喂食标准饲料多糖丰富（PR）日粮的B6品系悉生小鼠（gnotobiotic mice），经10天定植后采集其盲肠内容物样本，每组设置5个样本（n=5 samples/group），并对样本进行转录组谱分析。关键词：单菌定植vs.双菌定植，体内（in vivo）。从定植10天的悉生小鼠盲肠内容物中提取总RNA，随后将其与脆弱拟杆菌/长双歧杆菌基因芯片（GeneChip）进行杂交实验。