Lungs of ferrets infected with either an H3N2 or H5N1 subtype of influenza A virus.

A global genomics approach was used to identify patterns of immune dysregulation during H5N1 influenza virus infection as the host response, in particular hyperchemokinemia, is thought to contribute to the extreme pathology associated with this disease. Keywords: time course Ferrets were inoculated intranasally with 10(6) EID50 of either A/Vietnam/1203/04 (H5N1) or A/Panama/2007/99 (H3N2). At 2, 4 and 6 days post-infection (DPI), ferrets were euthanized and lung tissue was excised for RNA purification and subsequent gene expression analysis.

本研究采用全基因组学分析方法，旨在鉴定H5N1型流感病毒感染过程中的免疫失调模式。鉴于宿主应答（尤其是趋化因子血症（hyperchemokinemia））被认为是该疾病相关极端病理损伤的关键诱因，本研究围绕此展开。
关键词：时序动态（time course）
将雪貂经鼻内接种10⁶ 鸡胚半数感染量（EID₅₀）的A/Vietnam/1203/04（H5N1）或A/Panama/2007/99（H3N2）毒株。分别于感染后2、4、6天（DPI）安乐处死雪貂，摘取肺组织以进行RNA纯化及后续基因表达分析。