affy_med_2011_14-Transcriptomic analysis of roots of WT (A17) Medicago truncatula plants and of a hypermycorrhizal mutant (B9) grown on limiting (P/10) or non-limiting phosphate (P2).. Medicago truncatula

affy_med_2011_14 - affy_med_2011_14 - -In natural ecosystems most terrestrial plants form arbuscular mycorrhiza (AM), mutualistic symbioses with soil fungi belonging to the order Glomeromycota. This symbiosis takes place when phosphate is limiting and enables the plant to acquire nutrients (in particular phosphate) in exchange of carbon provided to the fungus A hypermycorrhizal mutant B9 was identified (Morandi et al. Mycorrhiza (2009) 19: 435) which displayed higher root colonization by Glomus intraradices. This phenotype could be linked to a defect in phosphate sensing or transport. We have undertaken the analysis of the root transcriptome of both WT and mutant plants grown on limiting or non-limiting phosphate to determine which processes are affected in the hypermycorrhizal mutant.-- Roots were harvested from 4 week-old WT and B9 mutant plants grown in a culture chamber with limiting (P/10, 130 µM phosphate) or non limiting (P2, 2.6 mM phosphate) phosphate under long days (16 hrs day at 22°C with 380 µE.m-2.s-1 light and 8hrs night at 19°C). RNAs were then extracted for transcriptomic analysis. We will compare WT (or mutant) root transcriptome on P/10 versus WT (or mutant) root transcriptome on P2 as well as mutant versus WT transcriptome on either P/10 or P2. Overall design: 12 arrays - Medicago; dose response,gene knock out

affy_med_2011_14 - affy_med_2011_14
在自然生态系统中，多数陆生植物可形成丛枝菌根（arbuscular mycorrhiza, AM）——即与球囊霉目（Glomeromycota）土壤真菌建立互利共生关系。该共生过程发生于磷酸盐受限条件下，植物可通过该共生体获取营养（尤以磷酸盐为甚），并向真菌供给碳源作为交换。
研究人员已鉴定出一株高菌根侵染突变体B9（Morandi等, *Mycorrhiza*, 2009, 19: 435），该突变体的根系被根内球囊霉（Glomus intraradices）的侵染水平显著更高。该表型可能与磷酸盐感知或转运功能缺陷相关。为明确高菌根侵染突变体中受扰动的生物学过程，本研究对生长于磷酸盐受限与非受限条件下的野生型（wild type, WT）及B9突变体植株的根系转录组进行了分析。
实验材料为培养箱中培养的4周龄野生型与B9突变体植株，培养条件为长日照模式：光照16小时（22℃，光照强度380 µE·m⁻²·s⁻¹）、黑暗8小时（19℃），分别设置磷酸盐受限（P/10，130 µM磷酸盐）与非受限（P2，2.6 mM磷酸盐）两个处理组，随后收取植株根系。提取根系总RNA用于转录组分析。本研究将开展两类比较：其一为同一基因型（野生型或突变体）在P/10与P2条件下的根系转录组差异；其二为相同磷酸盐处理条件（P/10或P2）下，突变体与野生型的根系转录组差异。
实验整体设计：共12张基因芯片，实验材料为苜蓿属（Medicago）植物，涵盖磷酸盐剂量响应与基因敲除相关实验设计。