Regional information in dental mesenchyme

Positional information plays a crucial role in embryonic pattern formation, yet its role in tooth development remains unexplored. In this study, we investigated the regional specification of lingual and buccal dental mesenchyme during tooth development. Overall design: Comparative gene expression profiling analysis of RNA-seq data between cap and bell stage, lingual and buccal part of dental mesenchyme at cap and bell stage, and lingual and buccal part of dental mesenchyme which were cultured in vitro at cap stage. - (Cap/Bell stage)_"W" means whole tooth germ - "L" means lingual - "B" means buccal

位置信息在胚胎模式形成中发挥关键作用，但其在牙齿发育中的作用仍未被探索。本研究针对牙齿发育过程中舌侧与颊侧牙间充质（dental mesenchyme）的区域特化展开了探究。整体实验设计：对帽状期（cap stage）与钟状期（bell stage）、帽状期及钟状期牙间充质的舌侧与颊侧部分，以及帽状期体外培养的牙间充质舌侧与颊侧部分的RNA测序（RNA-seq）数据开展比较基因表达谱分析。——（帽/钟状期）“W”代表完整牙胚（tooth germ），“L”代表舌侧（lingual），“B”代表颊侧（buccal）