Expression data from naive and memory CD8 T cells cultured in presence or absence of IL-4. Mus musculus

Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies suggest that IL-4 could also affect several functions of CD8 T cells such as cytotoxicity. Our team has shown that IL-4 repress the expression of Ccl5 in vitro. To define more precisely the impact of IL-4 on CD8 T cells, we performed a whole genome expression microarray analysis of naive and memory CD8 T cells cultured in presence or absence of IL-4. This approach allowed us to define the IL4-gene-expression signature on CD8 T cells. Overall design: 18 samples were processed. Two populations of F5 naive CD8 T cells were FACS-sorted: samples from each population were incubated 20 hours with IL-7 in presence or absence of IL-4. Thus, a total of 6 “Naive” samples were processed. In addition, 4 populations of F5 TIM memory CD8 T cells were FACS-sorted: samples from 2 of these populations were incubated 20 hours in presence of IL-7 and/or IL-4, or in medium alone. Thus, 12 “Memory” samples were processed.

白细胞介素4（IL-4）对CD8阳性T细胞功能的影响在很大程度上尚未明确。已有研究表明IL-4可诱导CD8阳性T细胞的存活与增殖，但也有多项研究提示IL-4还可能影响CD8阳性T细胞的多种功能，如细胞毒性。本团队已在体外实验中证实，IL-4可抑制CC趋化因子配体5（Ccl5）的表达。为更精准地阐明IL-4对CD8阳性T细胞的调控作用，本研究对在添加或不添加IL-4的条件下培养的初始型及记忆型CD8阳性T细胞开展了全基因组表达微阵列分析。通过该分析方法，我们明确了CD8阳性T细胞中的IL-4调控基因表达特征。
整体实验设计：共处理18份样本。首先通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）分选出两群F5初始型CD8阳性T细胞，将每群样本分别在添加白细胞介素7（IL-7）且存在或不存在IL-4的条件下孵育20小时，由此获得共计6份"初始型"样本。此外，通过荧光激活细胞分选术分选出4群F5 TIM记忆型CD8阳性T细胞，其中两群样本分别在仅添加IL-7、添加IL-7与IL-4组合，或仅使用培养基的条件下孵育20小时，由此获得共计12份"记忆型"样本。