Primers used for amplification of the tuf DNA barcode.

Two pairs of primer cocktails were used for universal amplification of the tuf barcode from all phytoplasma strains employed in this study in a nested PCR assay. Tuf 340/Tuf890 and Tuf400/Tuf835 primer cocktails were used in direct and nested PCR respectively. Each primer cocktail contained slightly different variants of the same primer mixed in equimolar amounts. The nucleotide sequences of the general sequencing primers M13F and T7 are underlined with a single and a double line, respectively. Primer positions correspond to the positions in the tuf gene of ‘Ca. P. asteris’ strain AY-WB (Genbank accession number CP000061).本研究针对所用全部植原体菌株的tuf条形码，通过嵌套PCR试验开展通用扩增，共使用两套引物混合液（primer cocktail）。其中，Tuf340/Tuf890与Tuf400/Tuf835引物混合液分别用于直接PCR与嵌套PCR反应。每套引物混合液均由等摩尔浓度混合的同一引物的不同变体组成。通用测序引物M13F与T7的核苷酸序列分别以单下划线和双下划线标注。引物的对应位置参照植原体暂定种‘Ca. P. asteris’菌株AY-WB的tuf基因（GenBank登录号CP000061）确定。