Figure 1

Fig 1. HEK293T cells transduced with T7 RNAP support T7 promoter-dependent expression. HEK293T cells were transduced with MIP-T7RNAP or LPCX(AB)-T7RNAP or with the empty vectors as controls. Cells were treated with antibiotics to kill untransduced cells and then were transfected in triplicates with a plasmid expressing luciferase under control of the T7 promoter. Luciferase activity was quantified the next day as described in the Methods section. RLU, relative lights units. A Student’s t-test was used to assess statistical significance; **, P < 0.01; ***, P < 0.001.

图1 转导了T7 RNA聚合酶（T7 RNAP）的HEK293T细胞可支持T7启动子依赖性基因表达。实验中将HEK293T细胞分别转导MIP-T7RNAP、LPCX(AB)-T7RNAP，或以空载体作为阴性对照。随后使用抗生素清除未成功转导的细胞，再将细胞以三重生物学重复的方式转染由T7启动子调控的荧光素酶表达质粒。次日按照方法部分所述流程定量检测荧光素酶活性，RLU（相对光单位）为检测指标。本研究采用学生t检验（Student’s t-test）进行统计学显著性分析；**表示P<0.01，***表示P<0.001。