The Chd4 helicase regulates chromatin accessibility and gene expression critical for β-cell function in vivo [RNA-seq]

To establish the role of Chd4 on controlling gene expression programs in the beta cell, we generated a tamoxifen-inducible, beta-cell-specific knockout mouse model; control (MIP-CreERT; Chd4flox/+;R26RtdTomato) and Chd4Db (MIP-CreERT; Chd4flox/flox;R26RtdTomato. Dispersed islets from tamoxifen treated control and Chd4Db mice were flow-sorted, and RNASeq performed.

为明确染色质解旋酶DNA结合蛋白4（Chd4）在β细胞中调控基因表达程序的功能，我们构建了他莫昔芬诱导型β细胞特异性敲除小鼠模型，分为对照组（MIP-CreERT; Chd4flox/+;R26RtdTomato）与Chd4Db组（基因型为MIP-CreERT; Chd4flox/flox;R26RtdTomato）。随后，我们对经他莫昔芬处理的对照组与Chd4Db小鼠的解离胰岛进行流式分选，并完成RNA测序（RNASeq）实验。