Gliding motility assay using 22063mM D2O in solution. Replicate 3/3. ROI 12/15.

• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules.
• The buffer used was PEM (also known as BRB80) with the inclusion of 22063mM D2O.
• Images were taken with an Andor Luca EMCCD camera on an Olympus IX-71 microscope using a 60x 1.42NA PlanApo objective.
• This fileset is replicate 3/3 and ROI 12/15.
• Images were taken to investigate speed variations of microtubules due to the inclusion of D2O in the motility assay.
• The temperature of the slide was held constant at 32°C by an objective heater. Early ROIs, i.e. 1-5, have slower giding speeds due to the increasing temperature of the slide which, was prepared at room temperature before observation. After ROI 5 the gliding speed stabilizes.
• Pixels lengths were measured to be 166nm/pixel using a stage micrometer.
• After the files have downloaded, a simple command line entry will convert the images to a movie. Mencoder must be installed. ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi
• Upload of these files were made possible through the figshare API.

• 本数据集采用驱动蛋白-1（kinesin-1）与罗丹明（rhodamine）标记的微管（microtubules）开展滑行运动检测实验（gliding motility assay）。
• 实验所用缓冲液（buffer）为PEM（亦称为BRB80），其中添加了22063mM重水（D₂O）。
• 图像采集基于奥林巴斯IX-71显微镜，搭配Andor Luca EMCCD相机，使用60× 1.42数值孔径（Numerical Aperture, NA）PlanApo物镜完成。
• 本文件集为第3组重复实验（共3组），且对应感兴趣区域（Region of Interest, ROI）12（共15个ROI）。
• 本次图像采集旨在探究运动检测实验中添加重水对微管滑行速度的影响。
• 通过物镜加热器将载玻片温度恒定维持在32℃。前5个感兴趣区域（ROI 1-5）的滑行速度较慢，这是因为载玻片在观测前于室温下制备，随观测进程温度逐渐升高；在ROI 5之后，滑行速度趋于稳定。
• 使用载物台测微尺（stage micrometer）标定得到像素长度为166nm/像素。
• 文件下载完成后，可通过简单的命令行指令将图像转换为视频，需提前安装Mencoder软件。命令示例：`ls > list.txt | mencoder mf://@list.txt -mf w=496:h=658:fps=30:type=png -ovc lavc -lavcopts vcodec=mpeg4:mbd=2:trell -o output.avi`
• 本数据集文件通过figshare应用程序编程接口（Application Programming Interface, API）完成上传。