Interleukin-6 from the Adipose Secretome Potentiate Differentiation of Adipose Progenitors Through Activation of Redox Signaling

<b>Supplementary Figure 1. Adipogenic Stimulus Increases the Number of Short Mitochondria.</b> (A) Box plot of mitochondrial length analyzed by category, short, intermediate, or elongated for APCs treated adipogenic stimulus or vehicle normalized to cell number per slide in mitotracker-stained APCs (B) Representative images of mitotracker stained APCs.<b>Supplementary Figure 2. Conditioned Media Treatment Increases Adipogenesis.</b> (A) Eluted Oil Red O absorbance for cells treated with CD CM or HFD CM from male subcutaneous adipose tissue explants or vehicle on day 7 of differentiation (n=4). * p ≤ 0.05.<b>Supplementary Figure 3. IL-6 and TNF Identified as Upstream Regulators </b>(A) Network of IL-6 as an upstream regulator of differentially expressed proteins found in our dataset in IPA. (B) Network of TNFa as an upstream regulator of differentially expressed proteins found in our dataset in IPA.<b>Supplementary Figure 4. IL-6 Treatment Increases Adipogenesis.</b> (A) Eluted Oil Red O absorbance from APCs treated with IL-6 [0.1, 1, 5 ng/mL] (n=4). * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001.<br>

<b>补充图1. 成脂刺激可增加短线粒体的数量</b>
(A) 按线粒体长度类别（短、中等或细长）划分的箱线图，数据来自经成脂刺激或溶剂处理的脂肪祖细胞（Adipose Progenitor Cells, APCs），以Mitotracker染色的脂肪祖细胞每张玻片的细胞数进行归一化
(B) Mitotracker染色的脂肪祖细胞代表性图像
<b>补充图2. 条件培养基处理可促进成脂分化</b>
(A) 分化第7天时，经雄性皮下脂肪组织外植体的正常饮食条件培养基（CD CM）、高脂饮食条件培养基（HFD CM）或溶剂处理的细胞的洗脱油红O吸光度（n=4）。* p ≤ 0.05
<b>补充图3. IL-6与TNF被鉴定为上游调控因子</b>
(A) 经Ingenuity通路分析（IPA）鉴定的、以IL-6作为上游调控因子的本数据集差异表达蛋白调控网络
(B) 经Ingenuity通路分析（IPA）鉴定的、以TNF-α作为上游调控因子的本数据集差异表达蛋白调控网络
<b>补充图4. IL-6处理可促进成脂分化</b>
(A) 经浓度为[0.1、1、5 ng/mL]的IL-6处理的脂肪祖细胞的洗脱油红O吸光度（n=4）。* p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001<br>