Identification of genes characteristic of committed preadipocyte fibroblasts. Mus musculus

In order to identify the molecular mechanisms controlling preadipocyte commitment we derived new sublines of Swiss 3T3 fibroblasts with varying potential for adipogenesis. Swiss 2, Swiss 8, Swiss 9, and Swiss 19 differentiate into fat cells while Swiss 3, Swiss 5, and Swiss 22 have a lower propensity to differentiate into fat cells. The overall goal was to identify genes whose expression in the fibroblast state correlates with the ultimate potential of the cells to undergo adipogenesis Overall design: 4 preadipose fibroblast cell lines (Swiss 2, Swiss 8, Swiss 9, and Swiss 19 in triplicate or duplicate) were compared to 3 non-adipogenic fibroblast cell lines (Swiss 3, Swiss 5, Swiss 22 in triplicate)

为解析调控前脂肪细胞定向定型的分子机制，本研究构建了成脂分化潜能各异的瑞士3T3成纤维细胞新亚系。其中，Swiss 2、Swiss 8、Swiss 9及Swiss 19可向脂肪细胞分化，而Swiss 3、Swiss 5与Swiss 22的成脂分化倾向较低。本研究的核心目标为筛选出在成纤维细胞状态下的表达水平与细胞最终成脂分化潜能相关的基因。实验整体设计：将4株成脂性成纤维细胞系（Swiss 2、Swiss 8、Swiss 9及Swiss 19，分别设置3次或2次生物学重复）与3株非成脂性成纤维细胞系（Swiss 3、Swiss 5、Swiss 22，均设置3次生物学重复）进行对照分析。