Data from: Signatures of north-eastern expansion and multiple refugia: Genomic phylogeography of the Pine Barrens Treefrog, Hyla andersonii (Anura: Hylidae)

Range fragmentation poses challenges for species persistence over time and
may be caused by both historical and contemporary processes. We combined
genomic data, phylogeographic model testing, and paleoclimate niche
modeling to infer the evolutionary history of the Pine Barrens Treefrog
(Hyla andersonii), a seepage bog specialist, in eastern North America to
better understand the historical context of its fragmented distribution.
We sampled H. andersonii populations across the three disjunct
regions of the species’ range: Alabama/Florida (AF), the Carolinas (CL),
and New Jersey (NJ). Phylogenetic relationships within H.
andersonii were consistent between the nuclear species tree and
mitochondrial analyses, indicating divergence between AF and CL/NJ
(Atlantic clade) ~0.9 million years ago and divergence of the NJ clade
~0.15 mya. Several predictions of northeastern expansion along the
Atlantic coast were supported by phylogeographic analyses. Model testing
using genome-wide SNP data and species distribution models both provided
evidence for multiple disjunct refugia. This comprehensive phylogeographic
study of H. andersonii demonstrates a long history of range fragmentation
within an endemic coastal plain species and highlights the influence of
historical climate change on the current distribution of species and their
genetic diversity.

范围破碎化长期以来对物种种群的存续构成严峻挑战，其成因可追溯至历史与当代两类过程。本研究整合基因组数据、系统地理模型检验与古气候生态位模拟，旨在重建北美东部渗流沼泽特化物种——松沼雨蛙（*Hyla andersonii*）的演化历史，以明晰其分布破碎化的历史背景。我们在该物种分布的三个间断分布区域内采集了安德森雨蛙种群样本：阿拉巴马州/佛罗里达州（AF）、卡罗来纳地区（CL）以及新泽西州（NJ）。安德森雨蛙的系统发育关系在核基因物种树与线粒体基因组分析中结果一致，结果显示AF种群与CL/NJ种群（大西洋支系）的分化时间约为0.9百万年前，而NJ支系的分化时间约为0.15百万年前。系统地理分析结果支持了该物种沿大西洋海岸向东北扩张的多项预测。基于全基因组单核苷酸多态性（SNP）数据的模型检验与物种分布模型均为多个间断避难所的存在提供了支持证据。这项针对安德森雨蛙的系统性系统地理研究，揭示了一种特有海岸平原物种长期存在的分布破碎化历史，并凸显了历史气候变化对物种种群当前分布格局及其遗传多样性的深刻影响。