Uremic pruritus patients serum DIA

Uremic pruritus (UP) is one of the common symptoms in patients with uremia, and its etiology and mechanism have not been fully understood. In view of the high incidence of UP, finding the specific changed proteins in blood of UP patients will help revealing the potential biological mechanisms of UP and explore biomarkers. In this study, LC-MS/MS based data independent acquisition (DIA) mode was used to analyze the serum samples of 54 UP patients (DKD-UP, HN-UP and GN-UP, n=18 for each), 18 uremic patients without pruritus (Negative) and 18 CKD patients without pruritus (CKD).

尿毒症瘙痒症（Uremic pruritus，UP）是尿毒症患者常见临床症状之一，其病因与发病机制尚未完全阐明。鉴于尿毒症瘙痒症高发，探寻患者血液中特异性差异表达蛋白，有助于揭示该病潜在生物学机制并筛选潜在生物标志物。本研究采用基于液相色谱-串联质谱（LC-MS/MS）的数据非依赖采集（data independent acquisition，DIA）模式，对54例尿毒症瘙痒症患者（其中DKD-UP、HN-UP、GN-UP各18例）、18例无瘙痒症状尿毒症患者（Negative组）以及18例无瘙痒症状慢性肾脏病（chronic kidney disease，CKD）患者的血清样本进行了分析。