Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and rea1 mutant total and polysome-bound Transcriptomes. Zea mays

Analysis of gene expression level. The hypothesis tested in the present study was that rea1 mutant influence the translation level of translation and nucleosome assembly associated genes. Results provide important information of the gene translation level regulation of ribosome proteins, elongation factors, histones and genes associated with other biological processes. Overall design: Endosperm total and polysome-bound mRNA profiles of 15DAP wild type (WT) and rea1 mutant were generated by deep sequencing, in triplicate, using Illumina Hiseq 2500 (Zea mays).

基因表达水平分析。本研究所验证的科学假说为：rea1突变体（rea1 mutant）可影响翻译相关基因与核小体组装相关基因的翻译水平。研究结果为核糖体蛋白、延伸因子、组蛋白及其他生物学过程相关基因的翻译水平调控提供了重要信息。实验整体设计：采用Illumina Hiseq 2500测序平台，对玉米（Zea mays）开花后15天（15DAP）的野生型（WT）与rea1突变体的胚乳总mRNA及多聚核糖体结合mRNA的表达谱进行深度测序，每组设置3次生物学重复。