<p>Baseline characteristics.</p>

Given the potential for mitochondrial medicine as a therapy in various illnesses, mitochondrial tests derived from blood samples have gained increasing value. The aim of this study was to perform an in-depth investigation of mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) of healthy adult participants to characterize mitochondrial respiration in health. Adult participants without acute illness were recruited. PBMCs were isolated and quantitative, real-time measurements of mitochondrial oxygen consumption rate were performed using the Seahorse XF Cell Mito Stress Test Kit with Seahorse XFe96 Analyzer. The study included 184 participants without previously diagnosed medical conditions. There was no association between mitochondrial respiration and sex and age groups (≤ 30 years vs. > 30 years). Maximal and Spare respirations were associated with body mass index (BMI). The findings of this study contribute to the growing body of knowledge on mitochondrial bioenergetics in healthy adults and provide further insights into its association with demographic and anthropometric factors.鉴于线粒体医学在多种疾病治疗中的应用潜力，基于血液样本的线粒体检测的价值日益凸显。本研究旨在对健康成年受试者的外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）的线粒体呼吸功能开展深入探究，以明确健康状态下的线粒体呼吸特征。研究招募了无急性疾病史的成年受试者，分离外周血单个核细胞后，采用Seahorse XF细胞线粒体压力检测试剂盒结合Seahorse XFe96分析仪，对线粒体耗氧率进行定量实时检测。本研究共纳入184名既往未被诊断出任何疾病的受试者。分析结果显示，线粒体呼吸功能与受试者性别及年龄组（≤30岁与＞30岁）无显著关联，而最大呼吸与储备呼吸能力与体质量指数（Body Mass Index, BMI）显著相关。本研究结果为健康成年人线粒体生物能学领域的现有研究补充了更多数据，并进一步揭示了线粒体呼吸功能与人口统计学及人体测量学因素之间的关联。