LDDN-0075935 treatment did not attenuate active, phosphorylated Src, Lyn, ERK, JNK, or p38 protein levels in BV2 cells.

Microglial BV2 cells were treated with vehicle (DMSO), 0.5nM, 5nM, 50nM, 0.5 μM, 5μM, and 50 μM LDDN-0075935 for 1h. Cells lysates were used for western-blot analyses with (B) anti-pLyn (Tyr 396), (C) anti-pSrc (Tyr 416), (D) anti-pERK, (E) anti-pJNK, and (F) anti-p-p38 antibodies with Lyn, Src, ERK2, JNK, and p38 antibodies as their respective loading controls. Optical densities from three independent experiments were graphed and averaged ± SD (*p<0.05vs. vehicle). (A) A representative western blot is shown.

将小胶质细胞BV2（Microglial BV2 cells）以赋形剂（vehicle，即二甲基亚砜DMSO）、0.5nM、5nM、50nM、0.5 μM、5μM及50 μM的LDDN-0075935处理1小时。收集细胞裂解物用于蛋白质印迹（western-blot）分析，分别使用(B)抗磷酸化Lyn（酪氨酸396位点）抗体、(C)抗磷酸化Src（酪氨酸416位点）抗体、(D)抗磷酸化ERK抗体、(E)抗磷酸化JNK抗体及(F)抗磷酸化p38抗体，并以Lyn、Src、ERK2、JNK及p38抗体作为各自的内参对照。对三次独立实验所得的光密度值进行绘图并计算平均值±标准差（*p<0.05 与赋形剂组相比）。(A) 展示了代表性的蛋白质印迹结果。