Examination of PGAM5 deletion in macrophages during early atherosclerosis

CX3CR1creER/WT PGAM5flox/flox R26-tdTomato Ldlr-/- mice or littermate control mice were fed a tamoxifen-enriched high fat diet for 4-weeks, then assessed for immune cell numbers in the blood by flow cytometry and for plaque formation in the aortic arch and heart sinus cross sections.将CX3CR1creER/WT PGAM5flox/flox R26-tdTomato Ldlr-/- 小鼠及其同窝对照小鼠以富含他莫昔芬的高脂饲料喂养4周，随后通过流式细胞术检测血液中的免疫细胞数量，并对主动脉弓与心脏窦部切片的斑块形成情况进行评估。