Genome-wide expression of mRNAs and microRNAs in response to ERI2 knockdown

Here we describe the biological properties of new member of the Enhancer of RNAi (ERI) subfamily of DEDDh nucleases in humans, called ERI-2 that is important for miRNA processing and decay for a large subset of eukaryotic miRNA transcripts. hERI-2 is a potent Mg2+ dependent 3'-5' exoribonuclease that also regulates the cell cycle and is a negative regulator of RISC. Overall design: miRNA-seq and RNA-seq in ERI2 KD and control samples

本研究阐述了人类DEDDh核酸酶（DEDDh nuclease）RNAi增强子（Enhancer of RNAi, ERI）亚家族的新型成员ERI-2的生物学特性。该蛋白对真核生物中大量miRNA转录本的miRNA加工与降解过程至关重要。hERI-2是一种高效的Mg²+依赖型3'-5'核糖核酸外切酶，同时可调控细胞周期，并作为RNA诱导沉默复合物（RNA-induced silencing complex, RISC）的负调控因子。实验整体设计：在ERI2基因敲减（knockdown, KD）样本与对照样本中开展miRNA测序与RNA测序。