Estradiol Induced Transcriptional Profile of EAAE DNA Binding Deficient ERa Uterus

To evaluate the ability of a DNA binding deficient ERa to mediate transcriptional responses in the mouse uterus, ovariectomized mice were injected with 100 ul of saline or 250 ng of estradiol (E2) in 100 ul saline, uterine tissue was collected 2 hours filllowing the injection, and RNA was isolated 3 mice each, WT saline, WT E2 2h, EAAE saline, EAAE 2h E2

为评估DNA结合缺陷型雌激素受体α（ERα）介导小鼠子宫内转录应答的能力，研究人员对去卵巢小鼠分别注射100微升生理盐水，或100微升生理盐水中添加250纳克雌二醇（E2）的溶液。于注射后2小时收集子宫组织，每组取3只小鼠的样本提取RNA，实验分组包括野生型生理盐水组（WT saline）、野生型雌二醇2小时组（WT E2 2h）、EAAE生理盐水组（EAAE saline）以及EAAE雌二醇2小时组（EAAE 2h E2）。