Expression profiling by high throughput sequencing to evaluate the effect of aroylated phenylenediamine HO53 on autophagy induction in mature human bronchial epithelial cells (BCi-NS1.1 cell line)

We used RNA sequencing to exploit molecular mechanisms behind HO53 induced autophagy in mature human bronchial epithelial cells (BCi-NS1.1 cell line). We performed transcriptome analysis of mature bronchial epithelial cells (BCi-NS1.1 cell line) treated with aroylated phenylenediamine HO53 for different time points 4 h, 8 h and 24 h and compared it to transcriptome of untreated cells collected at the same time points. A broad effect of HO53 on the gene expression in mature human lung epithelial cells (BCi-NS1.1) shows molecular signature for pathways involved in autophagy regulation. The expression pattern for selected genes was reconfirmed by qRT-PCR using SYBR Green reagent. Expression profiling by high throughput sequencing of HO53 treated vs untreated differentiated in air-liquid intephase (ALI) culture human bronchial epithelial cells (BCi-NS1.1 cell line)

本研究采用RNA测序（RNA sequencing）技术，探究HO53诱导成熟人类支气管上皮细胞（BCi-NS1.1细胞系）发生自噬的分子机制。我们对经芳酰基苯二胺HO53分别处理4小时、8小时及24小时的成熟支气管上皮细胞（BCi-NS1.1细胞系）开展转录组分析，并与同期收集的未处理细胞的转录组进行对照比较。HO53对成熟人类肺上皮细胞（BCi-NS1.1）的基因表达具有广泛调控效应，其分子特征提示该化合物可调控自噬相关通路。我们采用SYBR Green试剂通过实时定量逆转录聚合酶链反应（qRT-PCR），验证了部分候选基因的表达模式。本数据集通过高通量测序，分析了经气液界面（air-liquid interface, ALI）培养分化的人类支气管上皮细胞（BCi-NS1.1细胞系）在HO53处理与未处理状态下的表达谱。