Proteome of wild type and Nsun7 knockout mice round spermatids

Investigation of a new RNA-modification-based mechanism in mammalian spermatogenesis. We created a mice line devoid of testis-specific m5C RNA methyltransferase Nsun7. These mice demonstrated male infertility due to a peculiar defect in sperm tail assembly – mispositioning of the longitudinal columns of the fibrous sheath. The round spermatids proteomes of wild type and Nsun7 knockout mice were obtained to study the molecular pathways affected by NSUN7.

哺乳动物精子发生过程中基于RNA修饰的新型机制探究。我们构建了缺失睾丸特异性m5C RNA甲基转移酶Nsun7的小鼠品系。该品系小鼠因精子尾部组装存在特异缺陷——纤维鞘纵柱位置异常，表现出雄性不育表型。我们获取了野生型与Nsun7敲除小鼠的圆形精子细胞蛋白质组，以解析NSUN7所影响的分子通路。