Liu et al_lipidomics.xlsx

WT and KRASG12V-expressing MDCK cells were treated with glucose-free medium (4h), UGCG inhibtior DL-PDMP (24h), glycolysis inhibitor 2DG (24h) before harvested for shot-gun mass spectrometry-based lipid analysis, performed by Lipotype GmbH (Dresden, Germany). Three biological replicates were included for each condition. Data are represented as molar precentages of total lipids.

野生型（Wild Type, WT）MDCK细胞（Madin-Darby犬肾细胞）与表达KRASG12V的MDCK细胞经如下处理后收集：分别以无糖培养基培养4小时、UGCG抑制剂DL-PDMP培养24小时、糖酵解抑制剂2DG（2-脱氧葡萄糖）培养24小时，随后由德国德累斯顿的Lipotype有限公司开展基于鸟枪法质谱的脂质组学分析。各实验条件均设置三次生物学重复，实验数据以总脂质的摩尔百分比形式呈现。