Table 1 in Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases

Table 1. Quantification of DNA following extraction from stool samples (Cryptosporidium and Giardia) and white blood cell samples (Leishmania). This table presents the number of positive experiments and the mean number of molecular targets detected from four different samples for each parasite. The same quantity of each sample was tested with and without FastPrep disruption. Without disruptionAfter FastPrep disruptionPositive PCRMean quantity Cells/tubePositive PCRMean quantity Cells/tubep valueCryptosporidium parvum4/45474/419400.03Giardia intestinalis2/49.54/46360.005Leishmania infantum4/43094/4285NS表1 粪便样本（隐孢子虫属（Cryptosporidium）、贾第鞭毛虫属（Giardia））与白细胞样本（利什曼原虫属（Leishmania））的DNA提取后定量分析。本表格展示了每种寄生虫的4份独立样本的阳性实验次数，以及检测到的分子靶点平均数量。每份样本均采用等量试样，分别在未经FastPrep破碎处理与经FastPrep破碎处理的两种条件下开展检测。 | 研究对象 | 未经FastPrep破碎处理 | | 经FastPrep破碎处理 | | p值 | |-------------------------|----------------------|---|-------------------|---|-------------| | | 阳性PCR结果 | 每管细胞平均定量值 | 阳性PCR结果 | 每管细胞平均定量值 | | | 微小隐孢子虫（Cryptosporidium parvum） | 4/4 | 547 | 4/4 | 19400 | 0.03 | | 蓝氏贾第鞭毛虫（Giardia intestinalis） | 2/4 | 9.5 | 4/4 | 6360 | 0.005 | | 婴儿利什曼原虫（Leishmania infantum） | 4/4 | 3094 | 4/4 | 285 | 无统计学意义（NS） |