RAT-seq mapping of the FLI1 exonic circular RNA FECR1 target gene network in MDA-MB231 breast cancer cells. RAT-seq mapping of the FLI1 exonic circular RNA FECR1 target gene network in MDA-MB231 breast cancer cells

FECR1 is an exonic circular RNA of the proto-oncogene FLI1. FECR1 is identified as a molecular component in the FLI1 promoter chromatin complex. To study its function in breast cancer, we utilized a RNA reverse transcription-associated trap sequencing (RAT-Seq) approach to map the DNA binding targets in MDA-231 cells. Overall design: RAT-Seq was utilized to generate the database to map the regulatory element network of FECR1.

FECR1是原癌基因FLI1的外显子环状RNA。FECR1被鉴定为FLI1启动子染色质复合物中的分子组分。为探究其在乳腺癌中的功能，本研究采用RNA反转录关联捕获测序（RNA reverse transcription-associated trap sequencing, RAT-Seq）技术，在MDA-231细胞中绘制其DNA结合靶点图谱。实验整体设计：本研究借助RAT-Seq技术构建数据库，以绘制FECR1的调控元件网络。