mRNA sequence Analysis of Apocynin treated MKN45. mRNA sequence Analysis of Apocynin treated MKN45

Noxo1, a component of NADPH oxidase 1 (NOX1) complex, is upregulated in gastric cancer cells in a inflammation-dependent manner, and plays an important role in tumorigenesis (Oncogene, 33: 3820, 2014). To examine the mechanism of NOX1/ROS signaling in tumorigenesis, MKN45 gastric cancer cells were treated with apocynin, an inhibitor for NOX, and their gene expression was examined by RNA sequencing. Based on expression data, Sox2 was shown to be suppressed by apocynin, suggesting a role of Sox2 in a inflammation-associated gastric tumorigenesis. Overall design: Total mRNA expression profiles of Apocynin administrated MKN45 in 2 trials.

Noxo1作为NADPH氧化酶1（NADPH oxidase 1, NOX1）复合物的组成亚基，在胃癌细胞中以炎症依赖性方式表达上调，并在肿瘤发生进程中发挥关键作用（《Oncogene》，33卷：3820页，2014年）。为探究NOX1/活性氧（Reactive Oxygen Species, ROS）信号通路在肿瘤发生中的作用机制，研究人员采用NOX抑制剂夹竹桃麻素（apocynin）处理MKN45胃癌细胞，并通过RNA测序（RNA Sequencing）检测其基因表达水平。基于表达谱数据，研究证实Sox2的表达可被夹竹桃麻素抑制，这提示Sox2在炎症相关胃癌发生进程中具有潜在调控作用。整体实验设计：本研究包含2次独立重复实验，针对经夹竹桃麻素处理的MKN45细胞检测其总mRNA表达谱。