Stress-driven feedback regulation of multiple tolerant genes improves xylose fermentation of Saccharomyces cerevisiae

The modification of the The modification of the tolerance of xylose-fermenting yeast is an urgent issue for improving ethanol production. In this study, multiple genes involving in superoxide dismutase, glutathione biosynthesis, NADPH regeneration and acetic acid degradation were overexpressed using stress-induced promoters, which is selected from the transcriptome data. Stress-induced promoters were used to realize the feedback control of the tolerant genes, which can ultimately improve the tolerance and ethanol production. We reported the stress-induced promoters for overexpressing tolerant genes and increasing yeast tolerance in a feedback manner Overall design: Examine the transcriptome under non-stress condition (30°C, 2% glucose) and stress condition (36°C, 10% glucose) of an industrial Saccharomyces cerevisiae CY

木糖发酵酵母（xylose-fermenting yeast）的耐受性改造，是提升乙醇生产的紧迫课题。本研究通过从转录组数据（transcriptome data）中筛选得到的胁迫诱导型启动子（stress-induced promoters），对超氧化物歧化酶（superoxide dismutase）、谷胱甘肽生物合成（glutathione biosynthesis）、NADPH再生（NADPH regeneration）以及乙酸降解（acetic acid degradation）相关的多个基因进行过表达。利用胁迫诱导型启动子可实现耐受性基因的反馈调控，最终提升酵母耐受性与乙醇产量。本研究报道了可通过反馈方式过表达耐受性基因、增强酵母耐受性的胁迫诱导型启动子。总体实验设计：检测一株工业酿酒酵母（Saccharomyces cerevisiae）CY在非胁迫条件（30℃、2%葡萄糖）与胁迫条件（36℃、10%葡萄糖）下的转录组。