Genome-wide chromatin and gene expression profiling during memory formation and maintenance in adult mice.

<b>DESCRIPTION</b><br>qPCR was used to assess the enrichment of ChIP and MeDIP experiments and to validate the integrity of sequencing libraries<br><br><b>FILES</b><br>Validation of ChIP-seq library enrichment:<br><b>20130426-CA1-1h-K27ac-K79me3-K4me3-H3-lib_qPCR.xlsx</b> - validation of ChIP-seq libraries for samples from CA1 taken 1 hour after conditioning and immunoprecipitated for H3K27ac, H3K79me3, H3K4me3 or H3<br><b>20130715-ACCnaiveK76me3K27ac_qPCR.xlsx</b> - validation of ChIP-seq libraries for naive samples from ACC and immunoprecipitated for H3K76me3 or H3K27ac<br><b>20131125-CA1-Naive_qPCR.xlsx</b> - validation of ChIP-seq libraries for naive samples from CA1<br><b>20150122-CA1-K9ac_qPCR.xlsx</b> - validation of ChIP-seq libraries for naive samples from CA1 and immunoprecipitated for H3k9ac<br><br>Validation of MedIP-seq library enrichment:<br><b>20150729-MeDIP-LIB-ACC_qPCR.xlsx</b> - MedIP-seq library validated for genes Cobl, Loxhd1, Vrk1 and GAPDHenh (-)<br><b>20150802-ACC-lib_qPCR.xlsx</b> - MedIP-seq library validated for genes Nova1 and Reelin<br><br><b>DATA GENERATION</b><br>qPCR experiments were run on Roche LighCycler 480, and the machine was used to export the Ct values. The resulting output was imported into pyQPCR (version 0.10dev) using dilutions of inputs as standards, along with the following parameters:<br><br>Calculation type: Absolute quantification<br>Confidence interval: 90.00%<br>Machine: Roche LighCycler 480<br>Maximum E(Ct): 0.30<br>Minimum Ct: 35.00<br>

<b>说明</b><br>本数据集采用定量聚合酶链反应（qPCR）评估染色质免疫共沉淀（ChIP, Chromatin Immunoprecipitation）与甲基化DNA免疫共沉淀（MeDIP, Methylated DNA Immunoprecipitation）实验的富集度，并验证测序文库的完整性。<br><br><b>文件列表</b><br>ChIP-seq文库富集度验证：<br><b>20130426-CA1-1h-K27ac-K79me3-K4me3-H3-lib_qPCR.xlsx</b> - 针对条件刺激后1小时采集的CA1脑区样本的ChIP-seq文库富集度验证，该样本分别针对H3K27ac、H3K79me3、H3K4me3及组蛋白H3进行了免疫沉淀<br><b>20130715-ACCnaiveK76me3K27ac_qPCR.xlsx</b> - 针对前扣带回皮层（ACC, Anterior Cingulate Cortex）未处理样本的ChIP-seq文库富集度验证，该样本分别针对H3K76me3与H3K27ac进行了免疫沉淀<br><b>20131125-CA1-Naive_qPCR.xlsx</b> - 针对CA1脑区未处理样本的ChIP-seq文库富集度验证<br><b>20150122-CA1-K9ac_qPCR.xlsx</b> - 针对CA1脑区未处理样本的ChIP-seq文库富集度验证，该样本针对H3K9ac进行了免疫沉淀<br><br>MeDIP-seq文库富集度验证：<br><b>20150729-MeDIP-LIB-ACC_qPCR.xlsx</b> - 针对Cobl、Loxhd1、Vrk1及GAPDHenh（-）基因验证的MeDIP-seq文库<br><b>20150802-ACC-lib_qPCR.xlsx</b> - 针对Nova1与Reelin基因验证的MeDIP-seq文库<br><br><b>数据生成方法</b><br>本数据集的qPCR实验均在罗氏LightCycler 480仪器上完成，通过该仪器导出循环阈值（Ct值）；随后以输入样本的稀释梯度作为标准品，将所得结果导入pyQPCR（版本0.10dev）进行分析，所用参数如下：<br><br>计算类型：绝对定量<br>置信区间：90.00%<br>仪器型号：罗氏LightCycler 480<br>最大E(Ct)值：0.30<br>最小Ct值：35.00