Evaluation of sperm by flow cytometry and production of GFP-expressing and naked neck offspring from interspecific male guinea fowl chimeras.

The GFP and naked neck loci are heterozygous and therefore, one half of the offspring from the injected PGCs inherit the expressed alleles at these loci. The high rates of fertility indicate that the testis is highly colonized by chicken PGCs in some of the interspecific male guinea fowls. Hybrids appear in the offspring of roosters with lower rates of germline transmission. GFP transmission is calculated from all embryos evaluated including embryos at D7 and chicks at hatch. Naked neck transmission was evaluated in hatched chicks and the hybrid percentage was calculated as a percentage of all offspring including both chicks and hybrids.

绿色荧光蛋白（Green Fluorescent Protein，GFP）基因座与裸颈基因座均为杂合状态，因此经注射的鸡源原始生殖细胞（Primordial Germ Cells，PGCs）所产生的子代中，有一半将继承这两个基因座上的表达等位基因。较高的受精率表明，在部分种间雄性珍珠鸡体内，鸡源原始生殖细胞已高效定植于其睾丸组织中。种系传递率较低的公鸡所产生的子代中会出现杂交个体。绿色荧光蛋白传递率的统计涵盖所有被检测的样本，包括胚胎发育第7天的胚胎以及出壳雏鸡。裸颈性状传递率仅在出壳雏鸡中进行检测，而杂交个体占比则以所有子代（包括雏鸡与杂交个体）为基数进行计算。