CGH: HiPro_v_LoPro

Two soybean near-isogenic lines (NILs) differing in seed protein content were genotyped to determine differential genetic introgressions from the wild relative Glycine soja. The CGH comparison reveals loci that are differentially introgressed between the two lines. Comparison of HiPro (LD0-15154) and LoPro (LD0-15146) soybean NILs; the reported values show the log2 ratio of the normalized HiPro hybridization signal (Cy3) over the normalized LoPro hybridization signal (Cy5)

本研究对两个种子蛋白含量存在差异的大豆近等基因系（near-isogenic lines, NILs）进行基因分型，以解析其从野生近缘种野大豆（Glycine soja）中发生的差异性遗传渐渗事件。通过比较基因组杂交（Comparative Genomic Hybridization, CGH）比对，可鉴定出两个品系间存在差异性渐渗的基因座。本研究同时针对高蛋白（High Protein, HiPro，LD0-15154）与低蛋白（Low Protein, LoPro，LD0-15146）大豆近等基因系开展比对分析，所报告的数值为标准化后的高蛋白杂交信号（Cy3）与标准化后的低蛋白杂交信号（Cy5）的log2比值。