Transcriptional response to drug-induced oligomerization of the AIM2-like receptors IFI16, PYHIN1, and MNDA.

We explored ALR function in a ligand-independent manner using a drug-inducible dimerization system in which ALRs were tagged with two FV domains that rapidly oligomerize upon addition of the drug AP1 We found that oligomerization of IFI16, PYHIN1, or MNDA did not result in any significant gene expression changes. Total RNA was isolated from BFP, IFI16-2xFV, PYHIN1-2xFV, or MNDA-2xFV-expressing THP1 cells mock-treated or treated with 30nM AP1 dimerizer drug for 4 or 12 hours.

本研究借助药物诱导二聚化系统，以配体非依赖方式探究AIM2样受体（ALR）的功能：该系统中，ALR被标记有两个FV结构域（FV domain），在加入药物AP1后可快速发生寡聚化。我们发现，IFI16、PYHIN1或MNDA的寡聚化并未引发显著的基因表达变化。我们从分别表达BFP、IFI16-2xFV、PYHIN1-2xFV或MNDA-2xFV的THP1细胞中提取总RNA，这些细胞经mock处理（即空白对照处理），或用30nM的AP1二聚化药物处理4小时或12小时。