RNA-sequencing from fetal thymocytes and thymus, lung and bronchus epithelial cells.

To investigate the contributions of immune and stromal cells to the production of interferon in the thymus, we performed bulk RNA-sequencing on thymocytes, thymic epithelial cels, bronchial epithelial cells and lung epithelial cells. Overall design: Bulk RNA-seq profiling of magnetic bead enriched populations from select human fetal (pcw 16-18) anterior foregut-derived organs.

为探究免疫细胞与基质细胞对胸腺内干扰素生成的贡献，我们对胸腺细胞、胸腺上皮细胞、支气管上皮细胞及肺上皮细胞开展了批量RNA测序（bulk RNA-sequencing）。整体实验设计：对经磁珠富集的、取自选定人类胎儿（pcw 16-18，即受孕后16-18周）前肠前体来源器官的细胞群体进行批量RNA-seq转录组谱分析。